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Figure 1 | BMC Biotechnology

Figure 1

From: Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiaeoverexpressing alkaline phosphatase

Figure 1

Specific alkaline phosphatase activity in nmoles of product/mg of prot.*min (A) and estimation of integrated expression cassette copy number by dot-blot hybridization (B) in the S. cerevisiae transformants and control strain. BY4742 – recipient strain; BY4742/Pho8vac – recombinant strains bearing plasmid pUC57-delta1_2-ADHpr-PHO8-CYCt-kanMX. Alkaline phosphatase activity is shown in nmoles of product/mg of prot.*min. For dot-blot hybridization genomic DNA was isolated from WT and recombinant strains, diluted and loaded onto nitrocellulose film. Amounts of loaded DNA is shown under the photo. PHO8 gene was used as a probe.

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BMC Biotechnology

ISSN: 1472-6750

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