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Table 2 Apparent kinetic parameters of the free fusion proteins and their immobilized counterparts

From: CBD binding domain fused γ-lactamase from Sulfolobus solfataricusis an efficient catalyst for (-) γ-lactam production

Fusion protein

Km(mmol l-1)

Vmax(U mg-1)

k cat (s-1)

Cbd-Linker-Gla

14.3 ± 1.6

71.6 ± 4.2

8.8 × 104

Immobilized Cbd-Linker-Gla

34.0 ± 1.6

344.9 ± 31.2

4.2 × 105

Gla -Linker- Cbd

5.7 ± 0.1

151.5 ± 30.3

1.8 × 105

Immobilized Gla -Linker- Cbd

25.0 ± 1.6

381.6 ± 44.2

4.7 × 105

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BMC Biotechnology

ISSN: 1472-6750

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