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Table 1 Primers used in this study

From: CBD binding domain fused γ-lactamase from Sulfolobus solfataricusis an efficient catalyst for (-) γ-lactam production

Name

Sequence (5′ → 3′)a

Description

p1 forward

CGCCATGGATTTGAAGGTTGAATTCTAC

For construction of pETcbd

p2 reverse

CGGGATCCAGCGGAGCCAGCGGAGCC CACCGGGTTCTTTACCC

p3 forward

GCGGATCCATGGGAATTAAGTTACCCACATTG

For construction of pETcbdgla

p4 reverse

CGCTCGAGTTTTTTGATTCTCTCAAATACAT

p5 forward

CGCATATGGGAATTAAGTTACCCACATTG

For construction of pETgla

p6 reverse

GCGGATCCAGCGGAGCCAGCGGAGCCTTTTTTGATTCTCTCAAATACAT

 

p7 forward

GCGGATCCAATTTGAAGGTTGAATTCTACAAC

For construction of pETglacbd

p8 forward

CGCTCGAG CACCGGGTTCTTTACCC

 
  1. aRestriction sites are underlined, sequence encoding the linker peptide were in italics and in grey shade.
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BMC Biotechnology

ISSN: 1472-6750

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