Figure 6

Inhibitory effects of rCTB–YVAD on LPS-induced caspase-1 activation and IL-1β secretion in Caco-2 cells. Caco-2 cells were treated with 10 μg/ml LPS for 12 h. The cell lysates were incubated at 30°C for 2 h in the absence or presence of 50 μM rCTB-YVAD or rCTB. Caspase-1 activity was determined with a colorimetric assay (A). Caco-2 cells were treated with 50 μM rCTB–YVAD or rCTB in the presence of 10 μg/ml LPS. The concentrations of IL-1β in the supernatant were determined with an ELISA (B). Data are the means ± SEM of three separate experiments performed in triplicate. ^##P < 0.01 compared with the control, **P < 0.01 compared with LPS  +  PBS treatment.