Skip to main content
Figure 3 | BMC Biotechnology

Figure 3

From: Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus caseiinhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cells

Figure 3

Purification of rCTB–YVAD from the culture supernatant of L. casei transformed with pSCTB–YVAD. rCTB–YVAD was purified using its His-tag with a nickel-bound affinity resin. Purification was confirmed by SDS-PAGE followed by CBB staining (A) and immunoblotting with an antibody directed against CT (B). Data are representative of three separate experiments. The GM1-ganglioside-binding activities of purified rCTB–YVAD and rCTB were analyzed by GM1-ELISA. Data represent the means ± SEM of three separate experiments performed in triplicate (C). **P < 0.01 compared with PBS.

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us