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Table 3 Characteristics of endo-arabinanases from T. thermarum DSM 5069 and other microorganisms

From: Expression and characterization of a GH43 endo-arabinanase from Thermotoga thermarum

Strain

K m (mg ml−1)

V max (μmol ml−1 min−1)

k cat (s−1)

k cat /K m (ml mg−1 s−1)

Optimal temp (°C)

Reference

Thermotoga thermarum

a12.3 ± 0.15

b3.3 ± 0.12

c28.6 ± 0.88

a1,052.1 ± 12.7

b510.9 ± 17.5

c164.0 ± 4.6

a349.3 ± 4.2

b169.6 ± 5.9

c54.4 ± 1.6

a28.4 ± 0.4

b51.4 ± 0.1

c1.9 ± 0.0

75

This study

Caldicellulosiruptor saccharolyticus

b18.1

ND

b49.8

b2.8

75

[3]

Bacillus licheniformis

b19 ± 0.2

ND

b161 ± 1.2

b8.47 ± 0.16

35

[5]

Bacillus subtilis

a2.0 ± 0.24

a,d250 ± 1.2

ND

ND

50

[20]

Thermotoga petrophila

b19.9 ± 4.7

b478.2 ± 63.6

ND

ND

73

[1]

Aspergillus aculeatus

b66

11

ND

ND

50

[16]

  1. ND: not determined. Data were carried out with linear arabinan (a), debranched arabinan (b), or sugar beet arabinan (c) as substrate. Values shown were the mean of triplicate experiments, and the variation about the mean was below 5%. d: μmol mg−1 min−1.