Expression and characterization of a GH43 endo-arabinanase from Thermotoga thermarum

BMC Biotechnology

Table 2 Effects of cations and chemical reagents on the activity of purified Tth Abn

Cations^a/Chemical reagents^b	Residual activity (%)
Control	100 ± 0.6
Mg²⁺	99 ± 1.2
Zn²⁺	14 ± 0.3
Mn²⁺	128 ± 2.9
Ba²⁺	113 ± 3.6
Ca²⁺	113 ± 1.7
Al³⁺	0
Cu²⁺	0
Co²⁺	82 ± 1.7
Ni²⁺	31 ± 0.5
Tween 60	133 ± 2.5
Tris	41 ± 0.4
SDS	111 ± 1.8

^aFinal concentration, 1 mM. ^bFinal concentration, 0.05% Tween 60 and Tris, 0.1% SDS. Values shown were the mean of triplicate experiments, and the variation about the mean was below 5%.

ISSN: 1472-6750