Figure 1
From: Production of recombinant human annexin V by fed-batch cultivation
SDS-PAGE analysis of rhANXA5 expression in fed-batch cultivations. Recombinant E. coli BL21(DE3) cells were cultivated in semi-defined (SD) media for 30 h in fed-batch cultivations. Feeding started 4 h after the beginning of batch cultivation and rhANXA5 expression was induced after 18 h of cultivation by the addition of 1 mM IPTG to the cultures. M: Thermo Scientific™ Unstained Protein MW Marker; lane 1: sample collected immediately before IPTG induction (18 h culture); lanes 2–7: samples collected after IPTG induction from 20 h (lane 2), 22 h (lane 3), 24 h (lane 4), 26 h (lane 5), 28 h (lane 6), and 30 h (lane 7) cultures.