Table 2 Overview of predicted TANGO aggregation score, production yield and pull-down activity for SH2 domain constructs included in this study
From: Solubility of recombinant Src homology 2 domains expressed in E. colican be predicted by TANGO
Short name | Construct | TANGO predicted aggregation score | Production yield (mg/L bacterial culture) | pY-protein binding activity (pull-down) |
|---|---|---|---|---|
1-TD | TSAd-67-207-IVTD | 793 | 0,15 | ND |
2-TD | TSAd-90-207-IVTD | 774 | ND | ND |
3-RD | TSAd-67-188-PHRD | 791 | ND | ND |
4-AS | TSAd-81-193-PAAS | 771 | ND | ND |
5-RD | TSAd-90-188-PHRD | 773 | ─ | ND |
5-RD YT | TSAd-90-188-PHRD | 342 | ─ | ND |
5-RD GYT | TSAd-90-188-PHRD | 103 | 0,35 | ND |
5-AS | TSAd-90-188-PHRD | 772 | 0,37 | +++ |
5-AS YT | TSAd-90-188-PAAS | 339 | 0,42 | ND |
5-AS GYT | TSAd-90-188-PAAS | 102 | 0,48 | ++ |
mTSAd | mTSAd-111-210-AAAS | 745 | ND | ND |
rTSAd | rTSAd-111-209-AAAS | 744 | ND | +++* |
ALX | ALX-31-126-RPHRD | 63 | ND | ND |
ALX TFV | ALX-31-126-RPHRD | 488 | ND | ND |
Lck | Lck-124-228-RPHRD | 24 | 19,46 | +++* |