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Figure 4 | BMC Biotechnology

Figure 4

From: Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris: yield enhancement using disposable culture-bag and its application to site-specific chemical modifications

Figure 4

SDS-PAGE analysis of hFasRECD-T-Fc mediated co-immunoprecipitation of the purified hFasLECDs. a) Co-immunoprecipitation using wild-type hFasRECD-T-Fc [15]. Lanes: M, molecular-weight size-markers; a, NFG5-hFasLECD [10]; b, tag-free hFasLECD; c, N-Ethylmaleimide adduct of NFG1CG4-hFasLECD, d, SUNBRIGHT® ME-050MA adduct of NFG1CG4-hFasLECD; e, buffer alone. The bands in “FasR-Fc” labeled region were derived from wild-type hFasRECD-T-Fc sample. b) Comparison between wild-type hFasRECD-T-Fc (lanes a, c and e) and hFasRECD-T-Fc (N102Q, N120Q) mutant (lanes b, d and f). Lanes: M, molecular-weight size-markers; a and b, tag-free hFasLECD; c and d, N-Ethylmaleimide adduct of NFG1CG4-hFasLECD; e and f, SUNBRIGHT® ME-050MA adduct of NFG1CG4-hFasLECD. The bands in “FasR-Fc” labeled region were derived from hFasRECD-T-Fc samples.

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BMC Biotechnology

ISSN: 1472-6750

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