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Figure 3 | BMC Biotechnology

Figure 3

From: Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris: yield enhancement using disposable culture-bag and its application to site-specific chemical modifications

Figure 3

SDS-PAGE analysis of digestion of hFasRECD-T-Fc (N102Q, N120Q) mutant with thrombin and purification of hFasRECD part. Protein G-agarose column purified hFasRECD-T-Fc (N102Q, N120Q) mutant (470 μg) was digested with bovine thrombin (47 U) in 50 mM Tris-HCl (pH 7.5) at 20°C, and the liberated mutant hFasRECD part in the reaction mixture was further purified as described previously [15]. Lanes: M, molecular-weight size markers; a, before digestion; b and c, after digestion (b, 2 h; c, 4 h); d, after removal of Fc fragment by Protein G-agarose column; e, after fractionation by cation-exchange chromatography. Used column: Resource S 1 ml.

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BMC Biotechnology

ISSN: 1472-6750

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