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Table 3 Effects of different chemicals on the activity of the purified recombinant PelN*

From: Cloning, expression and characterization of a pectate lyase from Paenibacillus sp. 0602 in recombinant Escherichia coli

Substrate Relative activity ** K m V max k cat kcat/K m
  (%) (g L−1) (mol mg−1 min−1) (s−1) (L s−1 g−1)
PGA 100.0 ± 4.2 3.48 ± 0.35 4.12 ± 0.77 3.29 × 106 9.45 × 105
Pectin 20–34% 100.9 ± 6.1 15.06 ± 1.24 15.16 ± 0.31 1.21 × 107 8.03 × 105
  55–70% 74.3 ± 2.6 3.32 ± 0.07 1.15 ± 0.09 0.92 × 106 2.77 × 105
  1. *Data represent means ± SD (n = 3) relative to the reference samples;
  2. **The specific activity of PelN on PGA under the standard conditions was used as the reference (100%).