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Table 3 Effects of different chemicals on the activity of the purified recombinant PelN*

From: Cloning, expression and characterization of a pectate lyase from Paenibacillus sp. 0602 in recombinant Escherichia coli

Substrate

Relative activity **

K m

V max

k cat

kcat/K m

 

(%)

(g L−1)

(mol mg−1 min−1)

(s−1)

(L s−1 g−1)

PGA

100.0 ± 4.2

3.48 ± 0.35

4.12 ± 0.77

3.29 × 106

9.45 × 105

Pectin

20–34%

100.9 ± 6.1

15.06 ± 1.24

15.16 ± 0.31

1.21 × 107

8.03 × 105

 

55–70%

74.3 ± 2.6

3.32 ± 0.07

1.15 ± 0.09

0.92 × 106

2.77 × 105

  1. *Data represent means ± SD (n = 3) relative to the reference samples;
  2. **The specific activity of PelN on PGA under the standard conditions was used as the reference (100%).
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BMC Biotechnology

ISSN: 1472-6750

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