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Table 2 Effects of different chemicals on the activity of the purified recombinant PelN

From: Cloning, expression and characterization of a pectate lyase from Paenibacillus sp. 0602 in recombinant Escherichia coli

Chemicals Relative activity (%)*
Concentration (mM) 0.5 1.0
None 100 ± 1 100 ± 1
Ca2+ 729 ± 38 437 ± 1
Mg2+ 125 ± 11 99 ± 3
Mn2+ 75 ± 3 57 ± 5
Cu2+ 79 ± 3 104 ± 11
Zn2+ 116 ± 8 106 ± 7
Fe2+ 82 ± 7 57 ± 3
Ba2+ 12 ± 3 3 ± 0
K+ 119 ± 8 100 ± 4
EDTA nd nd
Triton X-100 102 ± 9 100 ± 8
Tween-20 98 ± 2 116 ± 11
  1. nd: no detectable activity.
  2. *The activity under the standard conditions without any chemicals was used as the reference (100%), and data represent means ± SD (n = 4) relative to the untreated reference samples;