Table 1 Primers used to assess and confirm the mutants for IDM- ply , IDM- lytA , IFD- ply , and IFD- lytA
Sequence (5′–3′) | Accession number | PCR product size (bp) | |||
|---|---|---|---|---|---|
D39 | IDM | IFD | |||
Pneumolysin | |||||
ply-P1 | CCTGTATATCAAGGAATTGGCTACC | CP000410.1 | |||
ply-P2 | GATAGAAGAGCCGGATCTAGCTCG | CP000410.1 | 1824 | ND | 670 |
ply-P1 | CCTGTATATCAAGGAATTGGCTACC | CP000410.1 | |||
pVA891-Ra | CGCTCATCGTCATCCTCGGC | AB057644.1 | 1582 | ||
ply-P2 | GATAGAAGAGCCGGATCTAGCTCG | CP000410.1 | |||
pVA891-Fa, | CCAGTAGTAGGTTGAGGCCGTTG | AB057644.1 | 1445 | ||
ply-1a | CCTTTGGCTTTATCAATCGCTTTATCG | CP000410.1 | |||
ply-1b | actcttcacttacatgcatgcCGTACGGTTTATGAAAAAACC | CP000410.1 | 1227 | ||
ply-2a | gcatgcatgtaagtgaagagtGCTCCGCTTCTTTCTTTCGAT | CP000410.1 | |||
ply-2b | TTACCTGTCGCCCTTGCTC | CP000410.1 | 1185 | ||
ply-F91 | CGTTTCATCAAAGAGGGTAA | CP000410.1 | |||
ply-R1070 | GCTGTAACCTTAGTCTCAAC | CP000410.1 | 980 | ||
ply-pbF | TAGCGATTTGGCTGTGAA | CP000410.1 | |||
ply-pbR | GCTGATTACCCTCTTTGA | CP000410.1 | 483 | ||
Autolysin | |||||
lytA-P1 | CAGTACTTAAAGCTATCCGACTCGGTTTAC | CP000410.1 | |||
lytA-P2 | GTCTGGGGTGTTATTGTAGATAGAATG | CP000410.1 | 1346 | ND | 616 |
lytA-s1 | CATGGCGCCTTCTTTAGCGTCTA | CP000410.1 | |||
lytA-s2 | GAGTTCATGACGGACTACCGCCT | CP000410.1 | 546 | ||
lytA-P1 | CAGTACTTAAAGCTATCCGACTCGGTTTAC | CP000410.1 | |||
pEVP3-Rb | GCCAGTTTGAGGGGACGACG | 1234 | |||
lytA-P2 | GTCTGGGGTGTTATTGTAGATAGAATG | CP000410.1 | |||
pEVP3-F b | TAACCTAACTCTCCGTCGC | 1513 | |||
lytA-1a | GTTCCTCATACTTCCCTTG | CP000410.1 | |||
lytA-1b | cccatccactaatgcggccgcATGCCTTTATCCAGTCAGCGG | CP000410.1 | 1331 | ||
lytA-2a | gcggccgcattagtggatgggATTCCCAGTTGAGTGTGCGTG | CP000410.1 | |||
lytA-2b | TGCCAGAACTCTTGCCACAG | CP000410.1 | 1199 | ||
Glucose kinase | |||||
gki-up | GGCATTGGAATGGGATCACC | CP000410.1 | |||
gki-dn | TCTCCCGCAGCTGACAC | CP000410.1 | 626 | 626 | 626 |
gki-F603 | TGCCGATGAATACGAAGG | CP000410.1 | |||
gki-R791 | CGATTGTTGATGGGTTTAG | CP000410.1 | 189 | ||