Skip to main content
Figure 6 | BMC Biotechnology

Figure 6

From: Differences in virulence of pneumolysin and autolysin mutants constructed by insertion duplication mutagenesis and in-frame deletion in Streptococcus pneumoniae

Figure 6

Validation of TGR of WT- ply from IDM- ply mutant. (A) Expression of WT-PLY in the 8 h, Day 1 and Day 2 IDM-ply culture by real-time PCR (B) ClaI- digested D39, IDM-ply and AG2, which was sensitive to erm and was obtained from Day 1 culture of IDM-ply mutant, were probed with a digoxigenin-labeled ply-pb in southern blot hybridization. (C) AG2 would produce PLY whose size was the same as PLY of D39 when incubating with a rabbit polyclonal PLY antibody.

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us