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Table 1 Fractional factorial screening design and responses

From: Experimental design approach in recombinant protein expression: determining medium composition and induction conditions for expression of pneumolysin from Streptococcus pneumoniae in Escherichia coliand preliminary purification process

Condition

Induction absorbance

[IPTG]

Expression temperature

[Yeast extract]

[Tryptone]

[Glucose]

[Glycerol]

[Kanamycin]

Cell growth

Hemolytic activity

Process time (*)

Productivity

 

Abs ind

mM

°C

g/L

g/L

g/L

% v/v

μg/mL

Abs

HU/mL

min

HU/mL/min

1

0.8 (-1)

0.1 (-1)

25 (-1)

5.0 (-1)

0 (-1)

1 (-1)

0.0 (-1)

10 (-1)

2.08

612

345

1.77

2

2.0 (+1)

0.1 (-1)

25 (-1)

5.0 (-1)

0 (-1)

10 (+1)

0.4 (+1)

50 (+1)

2.86

1171

450

2.60

3

0.8 (-1)

1.0 (+1)

25 (-1)

5.0 (-1)

10 (+1)

1 (-1)

0.4 (+1)

50 (+1)

2.62

1306

345

3.79

4

2.0 (+1)

1.0 (+1)

25 (-1)

5.0 (-1)

10 (+1)

10 (+1)

0.0 (-1)

10 (-1)

3.46

1306

425

3.07

5

0.8 (-1)

0.1 (-1)

37 (+1)

5.0 (-1)

10 (+1)

10 (+1)

0.4 (+1)

10 (-1)

3.18

678

340

1.99

6

2.0 (+1)

0.1 (-1)

37 (+1)

5.0 (-1)

10 (+1)

1 (-1)

0.0 (-1)

50 (+1)

6.42

1188

385

3.09

7

0.8 (-1)

1.0 (+1)

37 (+1)

5.0 (-1)

0 (-1)

10 (+1)

0.0 (-1)

50 (+1)

2.78

658

350

1.88

8

2.0 (+1)

1.0 (+1)

37 (+1)

5.0 (-1)

0 (-1)

1 (-1)

0.4 (+1)

10 (-1)

3.76

656

470

1.40

9

0.8 (-1)

0.1 (-1)

25 (-1)

23.6 (+1)

10 (+1)

10 (+1)

0.0 (-1)

50 (+1)

3.45

1446

345

4.19

10

2.0 (+1)

0.1 (-1)

25 (-1)

23.6 (+1)

10 (+1)

1 (-1)

0.4 (+1)

10 (-1)

4.73

1344

403

3.34

11

0.8 (-1)

1.0 (+1)

25 (-1)

23.6 (+1)

0 (-1)

10 (+1)

0.4 (+1)

10 (-1)

2.65

915

345

2.65

12

2.0 (+1)

1.0 (+1)

25 (-1)

23.6 (+1)

0 (-1)

1 (-1)

0.0 (-1)

50 (+1)

4.21

1146

420

2.73

13

0.8 (-1)

0.1 (-1)

37 (+1)

23.6 (+1)

0 (-1)

1 (-1)

0.4 (+1)

50 (+1)

4.09

639

335

1.91

14

2.0 (+1)

0.1 (-1)

37 (+1)

23.6 (+1)

0 (-1)

10 (+1)

0.0 (-1)

10 (-1)

5.47

1013

420

2.41

15

0.8 (-1)

1.0 (+1)

37 (+1)

23.6 (+1)

10 (+1)

1 (-1)

0.0 (-1)

10 (-1)

4.02

335

335

1.00

16

2.0 (+1)

1.0 (+1)

37 (+1)

23.6 (+1)

10 (+1)

10 (+1)

0.4 (+1)

50 (+1)

5.40

1353

420

3.22

17

1.4 (0)

0.55 (0)

31 (0)

14.3 (0)

5 (0)

5.5 (0)

0.2 (0)

30 (0)

3.60

1469

370

3.97

18

1.4 (0)

0.55 (0)

31 (0)

14.3 (0)

5 (0)

5.5 (0)

0.2 (0)

30 (0)

3.79

1557

370

4.21

19

1.4 (0)

0.55 (0)

31 (0)

14.3 (0)

5 (0)

5.5 (0)

0.2 (0)

30 (0)

3.73

1610

360

4.47

20

1.4 (0)

0.55 (0)

31 (0)

14.3 (0)

5 (0)

5.5 (0)

0.2 (0)

30 (0)

3.82

1615

350

4.61

21

1.4 (0)

0.55 (0)

31 (0)

14.3 (0)

5 (0)

5.5 (0)

0.2 (0)

30 (0)

3.82

1506

370

4.07

22

1.4 (0)

0.55 (0)

31 (0)

14.3 (0)

5 (0)

5.5 (0)

0.2 (0)

30 (0)

3.68

1507

370

4.07

23

1.4 (0)

0.55 (0)

31 (0)

14.3 (0)

5 (0)

5.5 (0)

0.2 (0)

30 (0)

3.24

1158

370

3.13

24

1.4 (0)

0.55 (0)

31 (0)

14.3 (0)

5 (0)

5.5 (0)

0.2 (0)

30 (0)

3.32

1263

370

3.41

  1. (*)Process time refers to culture time from the inoculum growth at 37°C to the end of 4 h of induction at different conditions defined by the experimental design.
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