Figure 7
From: A cyclic nucleotide sensitive promoter reporter system suitable for bacteria and plant cells
Flow chart showing a sample experimental plan. The flow of experiments show that by assessing the putative proteins of interest using the OPTXcGMPRE:LUC or alternatively the OPTXGARE:LUC reporter the researcher can determine the ability of each recombinant protein to induce cGMP or cAMP in vivo. The most promising candidates can be selected for further analysis as recombinant proteins using conventional kits. Once the proteins are functionally verified, mutagenesis of putative functional domains or residues can be made then the researcher can again utilize the OPTXcGMPRE:LUC or alternatively the OPTXGARE:LUC reporter system to determine the most promising candidates to pursue.