Figure 4
From: A cyclic nucleotide sensitive promoter reporter system suitable for bacteria and plant cells
Cyclic nucleotide induced luciferase activity in plant protoplasts. Arabidopsis leaf mesophyll protoplasts were transiently transfected with a promoter luciferase reporter construct and treated as described below. At least 3 biological replicates were completed on different days for each promoter treatment combination. (A) OPTX:LUC treated with 0.1 or 1 μM 8-bromo cGMP or dibutyryl cAMP (n = 8–9); (B) OPTX:LUC treated with 0.1 μM 8-bromo cGMP, 30 μM GA or 30 nM DEA/NONOate (n = 6); (C) OPTXGARE:LUC treated with 0.1 μM 8-bromo cGMP, 0.1 μM dibutyryl cAMP or 30 μM GA (n = 3). Luciferase was normalized against protein content and asterisks indicate treatments significantly different from the control (P < 0.05; one way ANOVA, Dunnett’s multiple comparison post-test).