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Table 2 Amino acid composition of the proteins of P. putida KT2440 at a dilution rate ( D ) of 0.2 h -1

From: Reconciling in vivo and in silico key biological parameters of Pseudomonas putidaKT2440 during growth on glucose under carbon-limited condition

Amino acid

Mol % (this study)

Mol % PpuMBEL1071[20]

Ala

12

10

Arg

5

6

Asx

10

9

Cys

 

2

Glx

12

10

Gly

10

12

His

2

2

Ile

4

5

Leu

10

8

Lys

5

6

Met

 

3

Phe

4

4

Pro

5

4

Ser

5

4

Thr

5

5

Trp

 

1

Tyr

3

3

Val

7

8

  1. Concentrations of cysteine, methionine, and tryptophan could not be identified in this assay and were assumed to have identical molar fractions as in the E. coli biomass [10].
  2. Asx = Asp + Asn; Glx = Glm + Gln.
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BMC Biotechnology

ISSN: 1472-6750

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