Figure 8

Oxidation of Evans Blue by laccase. Changes in the absorbance visible spectrum of Evans Blue dye (EB) during its oxidation by 20 mU T. villosa laccase (TvL). The inset shows the colorimetric (decolorizing) response obtained using 50 μM EB, 50 μM Methyl Orange (MO) and 200 μM Remazol Brilliant Blue (RBB) after 30 min of reaction with 10 mU TvL in triplicate (A). Correlation between concentration of EB and maximum peak absorbance (B). Oxidation velocities of EB by TvL (200 mU) measured by the decrease of absorbance at 605 nm using dye concentrations within the plate reader’s detection limit (C). Decolorization of EB (50 μM) as absorbance decrease/min using different amounts of TvL (D).