Figure 4
From: Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source
Growth behavior in SME medium with 0. 5% colloidal chitin at 90° C. (A) Growth curves of wild type P. furiosus (plus signs), mutant MUR27Pf (triangles) and mutant MUR23Pf (crossings) with the redesigned chitinase as a single enzyme. (B) Growth analysis of the Pyrococcus strains by quantification of the ATP level (left axis). In addition, the final acetate concentration in the medium was analyzed after 55 h incubation (right axis). (C) Documentation of the incubated bottles after x h incubation, P. furiosus wild type is shown on the left side and mutant MUR23Pf on the right side. (D) Phase contrast microscopic picture of the mutant strain MUR23Pf grown on chitin.