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Table 2 Crosses between ASAL and cre lines and estimation of the frequency of cre -mediated excision

From: Development of selectable marker free, insect resistant, transgenic mustard (Brassica juncea) plants using Cre/loxmediated recombination

Cross no.

T1parent

T1parent

Hybrid line ID

Total plants

ASALand crepositive plants

hptnegative plants

Recombination frequency (% )

1

LA2

CC3

LA2CC3

30

16

9

56.25

2

LB9

CD1

LB9CD1

35

18

9

50

3

LD3

CA2

LD3CA2

25

15

7

46.67

4

LF5

CE5

LF5CE5

30

17

5

29.41

5

CA2

LD3

CA2LD3

40

22

10

45.5

6

CC3

LA2

CC3LA2

25

16

6

37.5

7

CD1

LB9

CD1LB9

30

18

4

22.2

8

CE5

LF5

CE5LF5

40

21

6

28.5