Figure 5
Expression of ASAL in F 1 hybrid plants. A Western blot analysis of protein extracts from leaves of nine hpt –ve F1 hybrid mustard plants. Lane 1, ~ 1 μg purified native ASAL used as positive control; lane 2, crude protein from untransformed plant used as negative control; lanes 3 – 11, show crude protein of LA2CC3 3, 6, 9, 12, 14, 18, 23, 24, 27. B ELISA analysis for expression of ASAL in total soluble protein of those mentioned F1 hybrid lines respectively.