Figure 2
Molecular analysis of gene integration and expression in T 0 lines. A Southern blot analysis of genomic DNA from leaves of putative T0ASAL- lox- hpt - lox plants probed with [α-32P] dCTP labeled ASAL. Lane 1, 362 bp ASAL coding sequence was used as positive control; lane 2, untransformed genomic DNA as negative control; lanes 3–8, HindIII digested genomic DNA from six ASAL plants LA, LB, LC, LD, LE, LF. B Southern blot analysis of genomic DNA from leaves of putative T0cre-bar plants probed with [α-32P] dCTP labeled cre. Lane 9, 1.1 kb cre coding sequence as positive control; lane 10, untransformed genomic DNA as negative control; lanes 11–15, HindIII digested genomic DNA from five cre plants CA, CB, CC, CD, CE. Approximate molecular weight markers are indicated in the middle. C Western blots analysis of protein from four T0 Southern positive ASAL-lox-hpt-lox mustard plants. ~ 1 μg purified native ASAL used as positive control (lane 1) and crude protein from untransformed plant used as negative control (lane 2). Lane 3 – 6 crude protein from four transgenic mustard plants LA, LB, LD, and LF.