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Figure 1 | BMC Biotechnology

Figure 1

From: Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome

Figure 1

Assaying integrase activity in E.coli. A. The reporter plasmid used to assay activity of the integrases in E. coli. This plasmid, derived from pACYC184, contains lacZα gene encoding the LacZα peptide flanked by integrase attachment sites. The intact reporter plasmid confers β-galactisidase activity on a strain containing the ΔlacZM15 allele and therefore the colonies appear blue on agar containing presence of X-Gal and IPTG. Active integrase promotes site-specific recombination between the attP and attB sites resulting in deletion of the lacZα gene and the colonies appear white. B. The appearance of E.coli containing the reporter plasmid with or without an integrase expression plasmid in the presence of X-Gal and IPTG.

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BMC Biotechnology

ISSN: 1472-6750

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