Table 2 Strains and plasmids used and built in this work

Strains:

E. coli:

DH-5α

F- φ80d lacZΔM15Δ (lacZY-argF) U169 deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44l- thi-1 gyrA96 relA1

[37]

X. dendrorhous:

UCD 67-385

ATCC 24230, wild-type. Diploid strain [31].

ATCC

Xd_1H

(DHS3/dhs3^::hph). Heterozygote transformant derived from UCD 67–385 containing an allele of the DHS3 locus with a deletion and a hygromycin B resistance cassette.

This work

Xd_2H

(dhs3^::hph/dhs3^::hph). Homozygote transformant derived from Xd_1H by DRM [30] with a deletion and a hygromycin B resistance cassette in both alleles of the DHS3 locus.

This work

Xd_1H1S

(DHS3/dhs3^::hph+crtS). Heterozygote transformant derived from UCD 67–385 with a deletion in one DHS3 allele and bearing the hygromycin B resistance cassette and the crtS gene expression cassette.

This work

Xd_2H2S

(dhs3^::hph+crtS/dhs3^::hph+crtS). Homozygote transformant derived from Xd_1H1S by DRM with a deletion in both DHS3 alleles and bearing the hygromycin B resistance cassette and the crtS gene expression cassette.

This work

Plasmids:

pBluescript SK- (pBS)

ColE1 ori; AmpR; cloning vector for blue-white selection

Stratagene

pMN-hph

pBS containing a cassette of 1.8 kb bearing the resistance Hygromycin B (hph) gene under EF-1 α promoter and gpd transcription terminator of X. dendrorhous in its EcoRV site.

[30]

pXd_Ex_crtS

pBS containing the cDNA encoding the X. dendrorhous crtS gene in its EcoRV site.

[26]

pBS-PTEF-crtS-Tact

pBS containing a 2,367 bp cassette bearing the cDNA encoding the X. dendrorhous crtS gene under EF-1α promoter and the actin transcription terminator from X. dendrorhous in the EcoRV site.

This work