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Table 1 Viral IL-10 concentrations in different cell compartments of E. coli BL 21 (DE3)

From: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia colivia a modified Sec-dependent transporter construct

Viral IL-10 concentration (ng/ml ± SD)

HCMV IL-10 expression plasmid pAZ1c

EBV IL-10 expression plasmid pGA6

Supernatant

8.4 ± 12.4

1.2 ± n.d.

Periplasm

67.8 ± 24.9

1500 ± 841.4

Cytoplasm

18700 ± 12800

27.8 ± 43.4

  1. Recombinant vIL-10 concentrations from overnight cultures of E. coli BL 21 (DE3) transformed with either pAZ1c (HCMV IL-10) or pGA6 (EBV IL-10) are given for different bacterial compartments. Quantification of vIL-10 expression was accomplished by use of a commercial human IL-10 (for EBV IL-10) and an in-house sandwich ELISA (for HCMV IL-10), respectively. SD = standard deviation; n.d. = not done.
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BMC Biotechnology

ISSN: 1472-6750

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