Figure 5

Colony PCR screening to detect successful cloning of Mitf. Presence of the Mitf coding region inside the plasmid vector pBluescript II KS(+) in correct orientation detected by colony PCR and analytical gel electrophoresis. From 40 individually tested colonies, 39 were judged positive as evident from the amplification of a DNA fragment (expected size: 1621 bp). Colonies from the same E. coli strain transformed with plasmid DNA lacking the Mitf coding region served as negative control (NC).