Figure 1
From: Simultaneous display of two large proteins on the head and tail of bacteriophage lambda
Construction of phages displaying GFP on their surface. A. Fluorescent plaques were observed after cloning of the GFP in the λKM8 (top picture) and λKM10 (lower picture) allowing to generate fusions to the N- and C- termini of the gpD, respectively, and to display GFP on the lambda capsid. Empty clones after cloning were not fluorescent. The obtained phages were named GFP-N-λ and GFP-C-λ. B. Purification of GFP-C-λ (left tube) and wild type lambda (right tube) in CsCl gradient.