Figure 1

Efficiency of transfection reagents for mRNA delivery. (A) Diagram of a transposase vector plasmid and subsequent mRNA. The transposase gene sequence fused with the V5 epitope tag sequence (V5PB) and surrounded by the UTR5′ (U5) and UTR3′ (U3) sequences of the β-globin gene of the Xenopus laevis was cloned in the pCS2+ plasmid backbone to obtain the pCS2+ U5V5PBU3 construct. In vitro transcribed mRNA (V5PB mRNA) harbors a cap analogue (ARCA) and a polyA tail. (B) Efficiency of various different transfection reagents for mRNA delivery into HeLa cells. Cells were transfected with 200 ng of V5PB mRNA using jetPEI™ (N/P = 5), TransMessenger™ or TransIT®mRNA transfection reagents. Transfection efficacy was checked following protein expression by Western-Blot analysis 24 h post-transfection using an antiV5-Horseradish Peroxidase antibody to detect the tagged V5PB transposase (V5PB Tp). Protein quantification was normalized to the Menin endogenous protein (ratios are indicated). Data are the average of 4 experiments. Mock is a negative control without mRNA transfected.