Table 1 Screening of extracellular PCX cellulase activity of knockout and original S. cerevisiae BY4741 strains by 4-MUC assay (recombinant/original ratio)
Gene null mutant | ORF name a | 96-well screening 4-MUC assay | Condensed sample 4-MUC assay (o/n OD 600 ) |
|---|---|---|---|
Protein-trafficking genes: | |||
chc1∆::KanMX | YGL206C | 2.5 | 2.4 (2.8) |
clc1∆::KanMX | YGR167W | 3.8 | 2.6 (3.1) |
nyv1∆::KanMX | YLR093Cf | 1.5 | 1.7 (5.7) |
pep12∆::KanMX | YOR036W | 1.9 | 2.6 (2.9) |
vam3∆::KanMX | YOR106W | 1.6 | NA |
vps1∆::KanMX | YKR001C | 1.2 | NA |
vps15∆::KanMX | YBR097W | 1.9 | 1.3 (4.2) |
vps21∆::KanMX | YOR089Cf | 3.3 | 6.5 (6.1) |
vps33∆::KanMX | YLR396C | 1.5 | 0.9 (5.5) |
ypt7∆::KanMX | YML001Wb | 1.7 | 1.0 (5.1) |
Glycosylation-related genes: | |||
aga2∆::KanMX | YGL032C | 0.9 | NA |
alg3∆::KanMX | YBL082Cc | 0.5 | NA |
alg5∆::KanMX | YPL227Cc | 0.7 | NA |
alg6∆::KanMX | YOR002Wc | 0.8 | NA |
alg8∆::KanMX | YOR067Cc | 1.5 | NA |
alg9∆::KanMX | YNL219Cc | 1.5 | NA |
alg12∆::KanMX | YNR030Wc | 1.7 | NA |
anp1∆::KanMX | YEL036Cc | 1.8 | NA |
csg2∆::KanMX | YBR036C | 1.0 | NA |
cwh41∆::KanMX | YGL027Cc | 0.1 | NA |
cwh43∆::KanMX | YCR017C | 1.3 | NA |
die2∆::KanMX | YGR227Wc | 1.5 | NA |
gda1∆::KanMX | YEL042W | 1.6 | NA |
gnt1∆::KanMX | YOR320C | 1.2 | NA |
gon7∆::KanMX | YJL184W | 0.1 | NA |
gtb1∆::KanMX | YDR221Wd,e | 2.7 | 0.0 (12.6) |
hoc1∆::KanMX | YJR075W | 0.9 | NA |
hor7∆::KanMX | YMR251W-A | 1.4 | NA |
ktr1∆::KanMX | YOR099Wd | 0.8 | NA |
ktr2∆::KanMX | YKR061Wd,f | 0.9 | NA |
ktr3∆::KanMX | YBR205Wd | 1.0 | NA |
ktr4∆::KanMX | YBR199Wd | 1.0 | NA |
ktr5∆::KanMX | YNL029Cc | 1.0 | NA |
ktr6∆::KanMX | YPL053Cd | 1.3 | NA |
ktr7∆::KanMX | YIL085Cd | 1.0 | NA |
mnl1∆::KanMX | YHR204Wd | 1.2 | NA |
mnn2∆::KanMX | YBR015Cc | 1.3 | 0.8 (3.8) |
mnn5∆::KanMX | YJL186Wf | 1.9 | 3.0 (5.6) |
mnn9∆::KanMX | YPL050Cc,f | 2.2 | 3.8 (5.5) |
mnn10∆::KanMX | YDR245W | 2.5 | 6.0 (3.8) |
mnn11∆::KanMX | YJL183Wc | 2.0 | 4.5 (3.7) |
mns1∆::KanMX | YJR131Wd | 0.5 | NA |
mnt2∆::KanMX | YGL257Cd | 1.2 | NA |
mnt3∆::KanMX | YIL014Wd,e | 2.5 | 1.7 (5.2) |
mrl1∆::KanMX | YPR079Wf | 1.6 | NA |
ost3∆::KanMX | YOR085Wc | 1.1 | NA |
ost4∆::KanMX | YDL232Wc,f | 1.6 | NA |
ost5∆::KanMX | YGL226C-Ac | 0.9 | NA |
ost6∆::KanMX | YML019Wc,f | 1.8 | NA |
pmt1∆::KanMX | YDL095Wc | 3.1 | 3.2 (4.7) |
pmt2∆::KanMX | YAL023Cc | 5.3 | 1.8 (4.7) |
pmt3∆::KanMX | YOR321Wc | 1.1 | 1.0 (4.4) |
pmt5∆::KanMX | YDL093Wc | 2.1 | 4.3 (6.3) |
pmt6∆::KanMX | YGR199Wc | 0.8 | NA |
ynd1∆::KanMX | YER005Wf | 1.1 | NA |
yur1∆::KanMX | YJL139Cf | 2.0 | 2.8(4.5) |
yjr061w∆::KanMX | YJR061W | 1.0 | NA |