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Figure 6 | BMC Biotechnology

Figure 6

From: Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papayaL.)

Figure 6

Real-time PCR analysis of coat protein (CP) transgene in seed mixtures of ‘ Waimanalo ’ and ‘ SunUp ’ . A) Linear regression obtained with coat protein primer pair at 10, 1, 0.4%. B) Papain primer pair (Lanes 1: water; 2–5: ‘SunUp’: ‘Waimanalo’ seed mixtures in the ratio of 10, 1, 0.4, 0.2 and 0.1%; 6: ‘SunUp’; 7: ‘Waimanalo’). C) CP primer pair (Lanes 1–5: ‘SunUp’: ‘Waimanalo’ seed mixtures in the ratio of 10, 1, 0.4, 0.2 and 0.1%; 6: ‘SunUp’; 7: ‘Waimanalo’; 8: water). M: 1 kb DNA ladder (Fisher Scientific).

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BMC Biotechnology

ISSN: 1472-6750

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