Purification of recombinant septin rods from E. coli . A. Coomassie stained SDS-PAGE of the purified septin rod. B. Isoelectric focussing of purified septin rods (left panel) or SNAP tagged septin rods (right panel). C. PAGE separation of the excised band from the IEF for SNAP tagged septin rods. All four septins are present in the complex. D. Analytical size exclusion chromatography of purified septin rods. The rods elute in one prominent peak containing all four constituents. The single septins can be detected by Western blot using antibodies against Cdc3 and the attached 6his tag and S tag, respectively.