Table 5 Primers used in this study
From: Chromosomal evolution of Escherichia coli for the efficient production of lycopene
Primers | Sequence and purposea |
|---|---|
BLP1 | 5′- ATCGCCTGTATGAACCTG -3′, Diagnostic PCR in attPP21 integration |
BLP4 | 5′- TAGAACTACCACCTGACC -3′, Diagnostic PCR in attPP21 integration |
AHP2 | 5′- ACACTTAACGGCTGACATGG -3′, Diagnostic PCR in attPP21 integration |
AHP3 | 5′- AACGAGTATCGAGATGGCAC -3′, Diagnostic PCR in attPP21 integration |
CGA | 5′- TCAAGAATCTGGTGACCGAGGAG -3′, Diagnostic PCR in attPP21 integration |
CGB | 5′- ACGCCGCTTCAATGACGCTG -3′, Diagnostic PCR in attPP21 integration |
VCA1 | GTCGTCAGGCTACTGCGTATG. Diagnostic PCR in recA deletion |
VCA2 | CACGATCCAACAGGCGAG. Diagnostic PCR in recA deletion |
NDF | 5′- TGGTAATAATGGCTTCGTCTG -3′, qPCR for the minD gene |
NDR | 5′- GCGATAAAGATGCCCTCAC -3′, qPCR for the minD gene |
QCF | 5′- CCAGGAGGGATATTTGC -3′, qPCR for the crtI gene |
QCR | 5′- CAGGGAGTGGAACGAGAAG -3′, qPCR for the crtI gene |
wrbAR | 5′- CGGTCGACGCGTATCCTCCTGTTGAAGATTAG CCGTT -3′ |
yedeOF | 5′- GCGTCGACAGGAGAGATAAAATGTCGCTCGT TTGTTCT -3′ |
ydeOR | 5′- GGTCTCTGCAGTCAAATAGCTAAAGCATTCATCGT -3′ |
atpEF | 5′- GAGAGCTCAGGAGGACTGTCATGGAAAACCTGAAT -3′ |
atpER | 5′- CGGGGTACCTAAATAAAAGCAACGCTTACTACGC -3′ |
dxsF | 5′-CTGGGATCCAGGAGATCCGCTATGGATCTTTTATCAATACAGGAC-3′ |
dxsR | 5′- CCGGGTACCGCTGTCATGATCCAATTCCTTTATGT -3′ |
idiF | 5′-GAGGTACCAGGAGTTGTTCGATGTCCAACAATG-3′ |
idiR | 5′-GGCTCTAGATTATTTAAGCTGGGTAAATGCAG-3′ |
appYF | 5′-GCCTGCAGAGGAGGTGCAAGATGGATTATGT-3′ |
appYR | 5′-CCGCATGCTTATCAGTCAATTGTTTTG-3′ |
pckF | 5′-GGAATTCTCAATGCGCGTTAACAATGGTT-3′ |
pckR | 5′-CTCCCATGGTTACAGTTTCGGACCAGCC-3′ |
ppsF | 5′-GAGGTACCAGGAGTTGTTCGATGTCCAACAATG-3′ |
ppsR | 5′-CGTCCCGGGTTATTTCTTCAGTTCAGCCAGG-3′ |
rpoSF | 5′-GCCTCTAGAAGGAGCCACCTTATGAGTCAGAATAC-3′ |
rpoSR | 5′-GGTCGACTTACTCGCGGAACAGCGCTT-3′ |
ycgWF | 5′-CGGCATGCAGGAGATAACAAATGAAGTGGATAGT-3′ |
ycgWR | 5′-GCCAAGCTTTAGCATATCGAGCATATTT-3′ |
yjiDF | 5′-GGTCGACAGGAGGCGCAAAATGATGCGACAAT-3′ |
YJIDR | 5′-GGCTGCAGTTAGCTGACATTCTCCAGCG-3′ |
APFP | 5′- CTCCGTATAGAGTTCCATCGT -3′, Diagnostic PCR in the replacement of appY promoter |
APRP | 5′- GCCACATTTCTGGGCTACGAC -3′, Diagnostic PCR in the replacement of appY promoter |
IKFP | 5′- TGTTTATCAAGAGTGTCTGAGCGT -3′, Diagnostic PCR in the deletion of the iclR gene |
IKRP | 5′- CGTTTTCACCGCAAATACCG -3′, Diagnostic PCR in the deletion of the iclR gene |