Figure 4

Humanness of therapeutic antibodies. (A) T20 scores are shown for the indicated chain type for 22 therapeutic mouse antibodies that underwent humanization. Each circle is one antibody, and the lines connect a mouse antibody with its respective humanized antibody. The T20 score cutoff is indicated by the dashed line. (B- E) Shown are the T20 score (top), H-score (middle), and G-score (bottom) of 98 heavy chain full-length (B) and kappa light chain full-length (C) sequences for mouse, chimeric, humanized, and fully human therapeutic antibodies. Note that the H-score and G-score are scaled based on Z-scores. The T20 score of heavy chain framework only (D) and kappa light chain framework only (E) sequences are presented. Each antibody is a circle, and the average ± SD T20 score is provided for each group. The T20 score cutoff is indicated by the dashed line. Select groups of antibodies were compared using Student’s t-tests, and the P-values of significance are listed for each comparison.