Figure 9

Production supernatants and protein A purified samples of 20 different scFv-Fc antibodies. A) Crude supernatant (10 μL or 5 μL) from 5 to 6 days production sample were analyzed by SDS-PAGE under reducing conditions and coomassie (R250) staining. B) Samples of 1 μg scFv-Fc antibody were also analyzed after protein A affinity purification.