Expression of 20 different scFv-Fc antibodies from the HAL phage display antibody generation pipeline. A total of 20 different scFv antibody gene fragment isolated by phage display from the naïve antibody gene libraries HAL7/8 were subcloned into the vector pCSE2.5-hIgG1Fc-XP. The scFv-Fc antibodies were expressed after transient transfection in HEK293-6E cells in 25 mL scale and shake flasks. After 48 h one volume of fresh medium and 0.5% tryptone TN1 was supplemented. After 5 to 6 days supernatants were harvested and analyzed by human IgG/Fc capture ELISA. Standard deviations were calculated from a minimum of triplicate ELISA samples.