Figure 3
From: Generation of a tumor- and tissue-specific episomal non-viral vector system
Hepatoma specific transgene expression with pEPito-hCMV/AFP-oFLuc in vivo.NMRI nu/nu mice bearing subcutaneous HUH7 human hepatoma tumors were injected intravenously with LPEI polyplexes (N/P 6) formed either with pEPito-hCMV/AFP-oFLuc or pEPito-CMV-oFLuc at a dose of 2.5 mg/kg (n = 6 per group). Luciferase activity (RLU/mg tissue) is shown in lysates of lung, tumor and liver 24 h after transfection; grey bars: pEPito-CMV-oFLuc, full bars: pEPito-hCMV/AFP-oFLuc; n = 6; * p < 0.05 (U-test, Mann–Whitney; lung signal pEPito-hCMV/AFP-oFLuc vs. pEPito-CMV-oFLuc).