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Table 7 Effect of SCNT embryo selection on the cloning outcome

From: Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set

Selection timing1

Chance for pregnancy

Chance for delivery

Cloning efficiency (%)

No. of live cloned piglets

No. of healthy cloned piglets

No selection

1.1

1.9

4.5

3.4

1.4

Selection for 1 day

0.9

-

4.9

4.3

1.5

Selection for 2 days

0.6

4.0

6.8*

3.2

2.0

Mixed selection

1.8

4.0

3.5

2.6

1.3

  1. No selection was used as the reference category. For details on the statistical analysis and definition of the cloning benchmarks listed, please see Methods.
  2. * Statistically significant differences (p < 0.05).
  3. 1 No selection: all SCNT embryos transferred; selection for 1 day: 1-cell stage SCNT embryos transferred; selection for 2 days: 2-cell to 4-cell stage SCNT embryos transferred; mixed selection: mixed SCNT embryos transferred (no selection/1 day and 1 day/2 days)
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BMC Biotechnology

ISSN: 1472-6750

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