Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins

Table 2 Occurrence and size distribution of PBs in transgenic tobacco with different GFP-HFBI accumulation levels

Analyzed plants^a	GFP-HFBI Accumulation % TSP	Protein bodies	Protein body size
Analyzed plants^a	GFP-HFBI Accumulation % TSP	Absence^b	Presence	Small^c	Large^d
I64 GFP-HFBI	0.55%	0	100	97%	3%
I64 GFP-HFBI	1.68%	0	100	37%	63%
81V9 GFP-HFBI	0.77%	0	100	87%	13%
81V9 GFP-HFBI .	1.17%	0	100	48%	52%

^a, In total, five leaves per plant were sampled. Two leaf discs per leaf were collected and analyzed by laser scanning confocal microscopy. Ten cells were analyzed per leaf disc, for a total of 100 cells to avoid any bias.
^b, Cells displaying a bright fluorescent ER reticulated pattern.
^c, PB diameter <1.0 μm.
^d, PB diameter >1.0 μm.

ISSN: 1472-6750