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Table 2 Occurrence and size distribution of PBs in transgenic tobacco with different GFP-HFBI accumulation levels

From: Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins

Analyzed plantsa GFP-HFBI Accumulation % TSP Protein bodies Protein body size
Absenceb Presence Smallc Larged
I64 GFP-HFBI 0.55% 0 100 97% 3%
I64 GFP-HFBI 1.68% 0 100 37% 63%
81V9 GFP-HFBI 0.77% 0 100 87% 13%
81V9 GFP-HFBI . 1.17% 0 100 48% 52%
  1. a, In total, five leaves per plant were sampled. Two leaf discs per leaf were collected and analyzed by laser scanning confocal microscopy. Ten cells were analyzed per leaf disc, for a total of 100 cells to avoid any bias.
  2. b, Cells displaying a bright fluorescent ER reticulated pattern.
  3. c, PB diameter <1.0 μm.
  4. d, PB diameter >1.0 μm.