Functional expression of a blood tolerant laccase in Pichia pastoris

BMC Biotechnology

Table 3 I ₅₀ values (in mM) of sodium halides for ChU-B mutant produced in P. pastoris and S. cerevisiae

Inhibitor	Substrate	pH	ChU-B fromP. pastorisI₅₀(mM)	ChU-B from S. cerevisiaeI₅₀(mM)
NaF	ABTS	4.0	0.134	0.109
	ABTS	7.4 (blood buffer)	40	42
	DMP	5.0	0.174	0.183
NaCl	ABTS	4.0	1106	1025
	ABTS	7.4	n.m.	n.m.
	DMP	5.0	931	818

n.m. non-measurable. Inhibition studies were performed at the optimum pH activity value for each substrate tested (4.0 and 5.0 for ABTS and DMP, respectively) and at physiological pH (7.4) with ABTS as reducing substrate.

ISSN: 1472-6750