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Table 3 I 50 values (in mM) of sodium halides for ChU-B mutant produced in P. pastoris and S. cerevisiae

From: Functional expression of a blood tolerant laccase in Pichia pastoris

Inhibitor Substrate pH ChU-B fromP. pastorisI50(mM) ChU-B from S. cerevisiaeI50(mM)
NaF ABTS 4.0 0.134 0.109
ABTS 7.4 (blood buffer) 40 42
DMP 5.0 0.174 0.183
NaCl ABTS 4.0 1106 1025
ABTS 7.4 n.m. n.m.
DMP 5.0 931 818
  1. n.m. non-measurable. Inhibition studies were performed at the optimum pH activity value for each substrate tested (4.0 and 5.0 for ABTS and DMP, respectively) and at physiological pH (7.4) with ABTS as reducing substrate.