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Table 2 Biochemical characteristics of ChU-B mutant produced in P. pastoris and S. cerevisiae

From: Functional expression of a blood tolerant laccase in Pichia pastoris

Feature ChU-B fromP. pastoris ChU-B fromS. cerevisiae
Expression levels (mg/L) 43 8
MW1 53,939 53,939
MW2 62,541 60,310
Glycosylation (%) 16 12
Optimum pH (with ABTS) 4 4
Optimum pH (with DMP) 5 5
T50 (°C) 57.9 63.7
  1. 1Calculated from the amino acid composition ( 2Determined by MALDI-TOF mass spectrometry. The extra N-terminal extension is highlighted in bold.