Functional expression of a blood tolerant laccase in Pichia pastoris

BMC Biotechnology

Table 1 List of fungal laccases heterologously expressed in P. pastoris and S. cerevisiae

Heterologous host	Laccase source	Expression yields (mg/L)	Promoter	Reference
P. pastoris	Botrytis aclada ^a	517	GAP^c	[19]
P. pastoris	Botrytis aclada ^a	495	AOX1^d	[20]
P. pastoris	Pleurotus sajor-caju ^b	4.9	AOX1^d	[21]
P. pastoris	Pycnoporus cinnabarinus ^b	8	AOX1^d	[22]
P. pastoris	Trametes sp. 420^b	136	AOX1^d	[23]
P. pastoris	Trametes sp. AH28-2^b	31.6	AOX1^d	[24]
P. pastoris	Trametes trogii ^b	17	AOX1^d	[25]
S. cerevisiae	Melanocarpus albomyces ^a	7	GAL1^d	[26]
S. cerevisiae	Myceliophthora thermophila ^a,*	18	ADH1^c	[27]
S. cerevisiae	PM1^b,*	8	GAL1^d	[28]
S. cerevisiae	Pycnoporus cinnabarinus ^b,*	2	GAL1^d	[29]
S. cerevisiae	Trametes sp. C30^b	2	GAL10^d	[30]

^aAscomycete; ^bBasiodiomycete; ^cConstitutive promoter; ^dInducible promoter. ^*Laccase functional expression achieved by directed evolution.

ISSN: 1472-6750