Figure 6From: Influence of untranslated regions on retroviral mRNA transfer and expressionLuciferase activity upon retroviral transfer of mRNA into 293FT cells. 293FT cells were transduced with 100 μl supernatants containing retroviral particles harvested 48 h post-transfection. 3 or 6 h post-transduction, cells were lysed and luciferase activity was measured. We observed a statistically significant difference between the control condition 4 and all the test constructs (5 to 10) (p-value < 0,03). Statistical comparisons between the different constructs were performed on the 3 hours time-point and evaluated using a Wilcoxon-Mann–Whitney analysis. hptr, hours post-transfection. NT: untransfected cells. Number of experiments, N = 3.Back to article page