From: A mutant screening method by critical annealing temperature-PCR for site-directed mutagenesis
Primers | T c (°C) | Tested clones | Tc-PCR and sequence analysis | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Tc-PCR detect- able | Sequencing | Tc-PCR undetect-able | Sequencing | |||||||
Desired mut. | WT | Undesired mut. | Desired mut. | WT | Undesired mut. | |||||
D112K | 66 | 49 | 38 | 38 | 0 | 0 | 11 | 0 | 10 | 1 |
D112G | 68 | 50 | 26 | 26 | 0 | 0 | 24 | 0 | 23 | 1 |