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Table 3 T c -PCR screening and sequence analysis on clones of D112K and D112G

From: A mutant screening method by critical annealing temperature-PCR for site-directed mutagenesis

Primers T c (°C) Tested clones Tc-PCR and sequence analysis
Tc-PCR detect- able Sequencing Tc-PCR undetect-able Sequencing
Desired mut. WT Undesired mut. Desired mut. WT Undesired mut.
D112K 66 49 38 38 0 0 11 0 10 1
D112G 68 50 26 26 0 0 24 0 23 1