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Table 3 T c -PCR screening and sequence analysis on clones of D112K and D112G

From: A mutant screening method by critical annealing temperature-PCR for site-directed mutagenesis

Primers

T c (°C)

Tested clones

Tc-PCR and sequence analysis

Tc-PCR detect- able

Sequencing

Tc-PCR undetect-able

Sequencing

Desired mut.

WT

Undesired mut.

Desired mut.

WT

Undesired mut.

D112K

66

49

38

38

0

0

11

0

10

1

D112G

68

50

26

26

0

0

24

0

23

1