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Table 2 Comparison of induction stages for tyrosinase production

From: High level production of tyrosinase in recombinant Escherichia coli

Growth stage a μmax(h-1) b OD600maxc Max. act. (U mL-1) d
Early 0.39 ± 0.03 7.2 ± 0.5 0.05 ± 0.01
Mid 0.57 ± 0.02 8.4 ± 0.5 0.47 ± 0.07
Late 0.57 ± 0.03 8.9 ± 1.0 0.76 ± 0.06
  1. a. Cells were induced with 1 mM IPTG at the early-, mid- or late- growth phase.
  2. b. μmax: the maximal growth rate.
  3. c. OD600max: the maximal optical density measured at 600 nm.
  4. d. Max. act.: the maximal volumetric activity of tyrosinase in units per mL of culture broth.
  5. JM109 (pMFvpt) cells were grown in shake flasks at 37°C.