High level production of tyrosinase in recombinant Escherichia coli

BMC Biotechnology

Table 2 Comparison of induction stages for tyrosinase production

Growth stage ^a	μ_max(h^-1) ^b	OD_600max^c	Max. act. (U mL^-1) ^d
Early	0.39 ± 0.03	7.2 ± 0.5	0.05 ± 0.01
Mid	0.57 ± 0.02	8.4 ± 0.5	0.47 ± 0.07
Late	0.57 ± 0.03	8.9 ± 1.0	0.76 ± 0.06

^a. Cells were induced with 1 mM IPTG at the early-, mid- or late- growth phase.
^b. μ_max: the maximal growth rate.
^c. OD_600max: the maximal optical density measured at 600 nm.
^d. Max. act.: the maximal volumetric activity of tyrosinase in units per mL of culture broth.
JM109 (pMFvpt) cells were grown in shake flasks at 37°C.

ISSN: 1472-6750