Figure 4

(A) Cell sheet contraction under control, continued blebbistatin treatment (bleb+) or blebbistatin treatment for one day prior to dispase lifting, followed by reversion to normal media (bleb-) demonstrates that while blebbistatin does limit the degree of cell sheet contraction upon initial dispase lifting, the effects rapidly wear off and have no long-term effect on the degree of contraction. (B) Cell sheet cohesion is markedly reduced on blebbistatin treatment, but rapidly recovers on removal of blebbistatin. *p<0.05, **p<0.01 compared to the matched untreated control. Unnormalized times in seconds: control 0/1/2=93/253/208; bleb+ 0/1/2=23/41/11; bleb- 0/1/2=24/140/131.