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Figure 4 | BMC Biotechnology

Figure 4

From: Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from Burkholderia cenocepacia J2315 by phylogeny-based enzymatic substrate specificity prediction

Figure 4

Time course of the mandelonitrile hydrolysis mediated by the recombinant E. coli M15/BCJ2315. The reaction mixture (10 ml) containing wet cells (100 mg) and mandelonitrile (100 mM) suspended in phosphate buffer (100 mM, pH 8.0) was incubated in a rotary shaker (30°C, 200 rpm). Samples were taken every 10 min and quenched by the addition of 10% (v/v) 2 M HCl.

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BMC Biotechnology

ISSN: 1472-6750

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