Figure 6
From: An improved genetic system for bioengineering buoyant gas vesicle nanoparticles from Haloarchaea
Western blotting analysis of Halobacterium sp. NRC-1∆ ura 3∆ gvp C and derivative strains containing gvp C expression plasmids. Cell lysates (lanes 1–5) or gas vesicles (lanes 6–10) were electrophoresed on a 12% polyacrylamide-SDS gel, transferred to PVDF membrane, and probed with His-tag antibody followed by secondary antibody-alkaline phosphatase conjugate. Lanes 1 & 6: Halobacterium sp. NRC-1Δura3ΔgvpC, Lanes 2 & 7: Halobacterium sp. NRC-1Δura3ΔgvpC (pARK-C1), Lanes 3 & 8: Halobacterium sp. NRC-1Δura3ΔgvpC (pARK-C2), Lanes 4 & 9: Halobacterium sp. NRC-1Δura3ΔgvpC (pARK-C3), Lanes 5 & 10: Halobacterium sp. NRC-1Δura3ΔgvpC (pARK-C4).