Detection of luciferase activity by in-gel activity assays. Cell extracts of heat-shocked transformants were subjected to SDS-PAGE. Subsequently, in-gel renaturation was performed, the coelenterazine-substrate was added, and the gel was exposed to a light-sensitive film (right panels). As a loading control, the same extracts were also stained with Coomassie Blue following SDS-PAGE (left panels). (A) Analysis of transformants EuHR-1, EuHR-8, and EuHR-12, which were generated with pHRLucP as the co-bombarded plasmid. (B) Analysis of transformants EuHsp-2 and EuHsp-11, generated with pHsp70A-GLuc as the co-bombarded plasmid. In each experiment, the parental wild-type strain was analyzed as a control.